Gene expression construct of hap1 adapted for plant cells

Details Gene expression construct of hap1 adapted for plant cells Gene expression construct of hap1 adapted for plant cells

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2460632

Provided are novel nucleic acid molecule encoding modified HAP1 DNA-binding domains. Also provided are novel reporter genes based on GFP and extension. The materials provided by the invention may be used in a variety of methods of activating genes having HAP1 upstream activation sequences in plants, which methods can be used to co-ordinate or investigate gene expression, optionally in conjunction with GAL4-regulated expression, and also in novel "enhancer" traps.

L'invention se rapporte à une nouvelle molécule d'acide nucléique codant des domaines de liaison à l'ADN de HAP1 modifiés. Elle concerne aussi de nouveaux gènes rapporteurs fondés sur la protéine GFP et sur l'extensine. Les matériaux selon l'invention peuvent être utilisés dans plusieurs procédés d'activation de gènes présentant des séquences d'activation en amont de HAP1 dans les plantes. Lesdits procédés peuvent aussi servir à coordonner ou à rechercher l'expression de gènes, facultativement en parallèle avec l'expression régulée par GAL4, mais aussi être utilisés dans de nouveaux captages de <= séquences activatrices >=.

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