Gene expression profiling for identification, monitoring,...

Details Gene expression profiling for identification, monitoring,... Gene expression profiling for identification, monitoring,...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2670872

A method is provided in various embodiments for determining a profile data set for a subject with colorectal cancer or conditions related to colorectal cancer based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount of RNA corresponding to at least 1 constituent from Tables 1-5. The profile data set comprises the measure of each constituent, and amplification is performed under measurement conditions that are substantially repeatable.

L'invention concerne, dans divers modes de réalisation, un procédé de détermination d'un ensemble de données de profil chez un sujet souffrant d'un cancer colorectal ou d'un état pathologique associé au cancer colorectal, à partir d'un prélèvement effectué sur celui-ci, ce prélèvement constituant une source d'ARN. Le procédé consiste à utiliser l'amplifiation pour mesurer la quantité d'ARN correspondant à au moins un constituant des tables 1 à 5. L'ensemble de données de profil comprend la mesure de chaque constituant et l'amplification est réalisée dans des conditions de mesure pouvant être sensiblement reproduites.

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