Gene expression regulatory dna, expression cassette,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2261578

The present invention provides new gene expression regulatory DNA. This gene expression regulatory DNA comprises a promotor region derived from the barley D-hordein gene enabling the expression of a desired gene, and a regulatory region for regulating the expression of said gene based on said promoter region. This regulatory region consists at least of an activating region to activate the expression of said gene and a suppressing region to suppress the same. Use of this gene expression regulatory DNA enables controlling as desired the expression of gene within a plant cell such as barley.

La présente invention concerne un ADN régulateur de l'expression génique. Cet ADN régulateur de l'expression génique comporte un site promoteur, issu du gène D-hordéine de l'orge, et permettant l'expression du gène désiré, et un site régulateur permettant de réguler l'expression du gène considéré en fonction du site promoteur considéré. Ce site régulateur est constitué au moins d'un site activateur permettant d'activer l'expression du gène considéré et d'un site suppresseur permettant de supprimer l'expression du gène considéré. L'utilisation de cet ADN régulateur de l'expression génique permet de réguler comme on le souhaite l'expression du gène à l'intérieur d'une cellule de végétal tel que l'orge.

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