C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/18 (2006.01) C12N 15/28 (2006.01) C12N 15/37 (2006.01) C12N 15/67 (2006.01)
Patent
CA 2498776
The present invention discloses a method for modulating the production of a protein from a polynucleotide in a CHO cell by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower translation efficiency in the CHO cell than the codon it replaces, or by introducing into the CHO cell a polynucleotide that codes for an iso-tRNA which limits the rate of production of the polypeptide and which corresponds to a codon of the first polynucleotide. The present invention also discloses the use of a protein-encoding polynucleotide whose codon composition has been modified for enhanced production of the protein in CHO cells.
L'invention concerne une méthode de modulation de la production d'une protéine à partir d'un polynucléotide dans une cellule CHO. Cette méthode consiste à remplacer au moins un codon d'un polynucléotide par un codon synonyme présentant une efficacité de translation supérieure ou inférieure dans la cellule CHO, par rapport au codon qu'il remplace, ou à introduire dans la cellule CHO, un polynucléotide codant pour un iso-ARNt limitant la vitesse de production du polynucléotide et correspondant à un codon du premier polynucléotide. L'invention concerne également l'utilisation d'un polynucléotide codant une protéine dont la composition de codon a été modifiée pour une production accrue de la protéine dans des cellules CHO.
Fetherstonhaugh & Co.
The University Of Queensland
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