Gene expression technique

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/80 (2006.01) C12N 5/10 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2551503

The present invention provides a method for producing heterologous protein comprising: (a) providing a host cell comprising a 2~m-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (b) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (c) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium.

La présente invention concerne un procédé pour produire une protéine hétérologue, qui consiste (a) à disposer d'une cellule hôte comprenant un plasmide de la famille 2 µm qui comprend un gène codant une protéine qui présente la séquence d'une protéine chaperon et un gène codant une protéine hétérologue, (b) à mettre en culture la cellule hôte dans un milieu de culture dans des conditions qui permettent l'expression du gène codant la protéine chaperon et du gène codant une protéine hétérologue, puis (c) à purifier la protéine hétérologue ainsi exprimée à partir de la cellule hôte mise en culture ou du milieu de culture.

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