Gene imparting flocculability to yeast and gene product

Details Gene imparting flocculability to yeast and gene product Gene imparting flocculability to yeast and gene product

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C07K 14/395 (2006.01) C12C 11/02 (2006.01) C12N 1/19 (2006.01)

Patent

CA 2186875

The present invention relates to a protein having an activity of conferring on yeast brewer's yeast-type flocculating property; a DNA coding for the protein; a plasmid containing the DNA; a method for producing a yeast strain wherein brewer's yeast-type flocculating property has been conferred or enhanced with the use of the DNA and a method for producing a yeast strain wherein brewer's yeast-type flocculating property has been eliminated or reduced with the use of the DNA; and a method for eliminating or reducing brewer's yeast-type flocculating property of yeast by inhibiting the expression of the DNA. The present invention also relates to a yeast strain wherein brewer's yeast-type flocculating property has been conferred, enhanced, eliminated or reduced by the above method. Further, the present invention relates to a method for producing a brewed product comprising culturing the above yeast strain as well as a brewed product obtained by the method. According to.the present invention, there are provided the Lg-Flo1 protein having an activity of conferring on yeast brewer's yeast-type flocculating property and the DNA strand of Lg- FL01 coding for the protein. By introducing the above DNA of the invention into yeast cells as a foreign gene by genetic engineering techniques, i.e., introducing the DNA into yeast cells as an extranuclear gene and(/or) a nuclear gene, it is possible to confer on yeast brewer's yeast-type flocculating property or enhance this property in yeast. On the contrary, by introducing a DNA is obtained by destroying the DNA of the invention into yeast cells, or by inhibiting the expression of the DNA of the invention, it is possible to convert a flocculent yeast strain into a non-flocculent strain, or to reduce flocculating property.

L'invention concerne une protéine présentant l'activité de conférer une capacité de floculation de type levure de bière à des levures; un ADN codant cette protéine; un plasmide contenant cet ADN; un procédé de production d'une levure à laquelle on a conféré ou dont on a amélioré la capacité de floculation de type levure de bière à l'aide de l'ADN précité; un procédé de production d'une levure dont on a éliminé ou réduit la capacité de floculation de type levure de bière; ainsi qu'un procédé d'élimination ou de réduction de la capacité de floculation de type levure de bière d'une levure par suppression d'une expression de l'ADN précité; des levures auxquelles on a conféré une capacité de floculation de type levure de bière, laquelle a été améliorée, éliminée ou réduite par le procédé précité; un procédé de production de produits brassés, lequel consiste à incuber les levures précitées; ainsi que les produits brassés ainsi obtenus. L'invention concerne une protéine Lg-Flo1 et un ADN du gène Lg-FLO1 codant cette protéine. L'introduction de cet ADN en tant que gène étranger dans une levure par une technique de génie génétique, c'est-à-dire, l'introduction de cet ADN sous la forme de gènes extra-nucléaires et (ou) nucléaires dans des cellules de levure permet de conférer la capacité de floculation de type levure de bière à la levure ou d'en améliorer la capacité de floculation de type levure de bière. A l'inverse, une levure apte à la floculation peut être convertie en une levure non apte à la floculation ou sa capacité de floculation peut être réduite par introduction de cet ADN à l'état rompu, ou la suppression de l'expression de l'ADN.

Affiliated with

Also associated with

Rating

Gene imparting flocculability to yeast and gene product does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gene imparting flocculability to yeast and gene product, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene imparting flocculability to yeast and gene product will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1655509