Gene integration into chromosomes of lactobacillus...

Details Gene integration into chromosomes of lactobacillus... Gene integration into chromosomes of lactobacillus...

C - Chemistry, Metallurgy

12

N

C12N 15/75 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01) C12P 7/56 (2006.01)

Patent

CA 2117183

A B S T R A C T A gene to be integrated is bound to pAM.beta.1 plasmid to construct an integration plasmid, which is conjugally transferred into chromosomal DNA of a strain of Lactobacillus delbrueckii species. Integration of only the objective gene occurs through homologous recombination events (double crossing-over). According to this method, an arbitrary gene can be integrated into chromosomal DNA of a strain of Lactobacillus delbrueckii species and the DNA sequence derived from the vector, pAM.beta.1, can be completely removed. Therefore, the obtained gene integrant is widely applicable to the production of foods, medicines, feeds and the like. 51

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