Gene knockout mesophilic and thermophilic organisms, and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) C07H 21/02 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12N 9/90 (2006.01) C12N 9/92 (2006.01) C12N 15/00 (2006.01) C12P 7/54 (2006.01) C12P 7/56 (2006.01)

Patent

CA 2743505

One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non- native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

Un aspect de la présente invention concerne un micro-organisme thermophile ou mésophile génétiquement modifié, où un premier gène natif est partiellement, sensiblement, ou totalement délété, silencé, inactivé ou régulé à la baisse, ledit premier gène natif codant pour une première enzyme native impliquée dans la production métabolique dun acide organique ou dun sel de celui-ci, de manière à augmenter la capacité native dudit micro-organisme thermophile ou mésophile à produire du lactate ou de lacétate en tant que produit de fermentation. Dans certains modes de réalisation, le micro-organisme mentionné ci-dessus comprend en outre un premier gène non natif, ledit premier gène non natif codant pour une première enzyme non native impliquée dans la production métabolique de lactate ou dacétate. Un autre aspect de linvention concerne un procédé pour convertir une biomasse lignocellulosique en lactate ou en acétate, comprenant la mise en contact de biomasse lignocellulosique avec un micro-organisme thermophile ou mésophile génétiquement modifié.

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