Gene markers linked to fusarium head blight-resistance...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) A01H 5/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2553757

QTL analysis on the resistance to Fusarium head blight is performed by using an RI strain (RHI population) grown by crossing Russia 6 (two-row, resistant) with H.E.S.4 (six-row, susceptible), an RI strain (RI2 population) grown by crossing Harbin 2-row (resistant) with Turkey 6 (susceptible), and a DH strain (DHHS population) grown by crossing Haruna two-row (resistance) with H602 (susceptible). As a result, QTLs are detected on the 2H, 4H and 5H chromosomes in the RHI population, on the 2H, 4H and 6H chromosomes in the RI2 population and on the 2H, 4Hand 5H chromosomes in the DHHS population. Furthermore, gene markers linked to these QTLs (MM314, FM677, FM426, MM1057, MMtgaEatc128, FMgcgEatc530, FXLRRfor_XLRRrev119, FMacgEcgt288, HVM67, FMataEagc408, HVM11, Bmag125, k04002, k05042, k03289, HvLOX and k00835) are found out.

Selon l'invention, une analyse de QTL sur la résistance à la fusariose est effectuée au moyen d'une souche RI (population RHI) formée par croisement de Russie 6 (deux colonnes, résistant) avec H.E.S.4 (six colonnes, sensible), d'une souche RI (population RI2) formée par croisement de Harbin (deux colonnes, résistant) avec Turquie 6 (sensible), et d'une souche DH (population DHHS) formée par croisement de Haruna (deux colonnes, résistant) avec H602 (sensible). Les QTL sont ainsi détectés sur les chromosomes 2H, 4H, et 5H dans la population RHI ; sur les chromosomes 2H, 4H, et 6H dans la population RI2 ; et sur les chromosomes 2H, 4H, et 5H dans la population DHHS. En outre, les marqueurs génétiques liés à ces QTL (MM314, FM677, FM426, MM1057, MmtgaEatc128, FmcgcEatc530, FXLRRfor_XLRRrev119, FmacgEcgt288, HVM67, FmataEagc408, HVM11, Bmag125, k04002, k05042, k03289, HvLOX et k00835) sont découverts.

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