Gene markers useful for detecting skin damage in response to...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2423247

The cellular response to ultraviolet radiation exposure has been characterized on the molecular level through the use of high density gene array technology. Nucleic acid molecules and protein molecules, the expression of which are repressed or induced in response to ultraviolet radiation exposure, are identified according to a temporal pattern of altered expression post ultraviolet radiation exposure. Methods are disclosed that utilized these ultraviolet radiation-regulated molecules as markers for ultraviolet radiation exposure. Other screening methods of the invention are designed for the identification of compounds that modulate the response of a cell to ultraviolet radiation exposure. The invention also provides compositions useful for drug screening or pharmaceuticals purposes.

La réponse cellulaire à l'exposition à un rayonnement ultraviolet a été caractérisée au niveau moléculaire au moyen d'une technologie de réseaux de gènes haute densité. Des molécules d'acide nucléique et des molécules protéiniques dont l'expression est réprimée ou induite en réponse à l'exposition à un rayonnement ultraviolet sont identifiées en fonction d'un motif temporel d'expression modifiée après exposition à un rayonnement ultraviolet. Des procédés décrits utilisent ces molécules régulées par le rayonnement ultraviolet comme marqueurs d'exposition à un rayonnement ultraviolet. L'invention concerne aussi d'autres procédés de criblage permettant d'identifier des composés qui modulent la réponse d'une cellule à l'exposition à un rayonnement ultraviolet ; et des compositions utiles dans le criblage de médicaments ou à des fins pharmaceutiques.

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