Gene reporter assay, kit, and cells for determining the...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01)

Patent

CA 2500863

The present invention relates to a commercializable cell and to a gene reporter assay method and a kit which use this cell to determine the presence and/or the level of a molecule that activates signal transduction activity of a cell surface protein. This cell is treated in such a manner that it will have a sufficiently long shelf life for its intended purpose, whereupon at the end of its useful shelf life or at the end of its use, i.e., in an assay, the cell undergoes cellular death.

L'invention concerne une cellule pouvant être commercialisée et une méthode d'analyse de gène rapporteur et un kit faisant appel à cette cellule pour déterminer la présence et/ou le niveau d'une molécule qui active l'activité de transduction du signal d'une protéine de surface cellulaire. Cette cellule est traitée de façon que sa durée de vie soit suffisamment longue pour lui permettre d'exercer sa fonction, et que, à la fin de sa vie ou de sa durée d'utilisation, c'est-à-dire, à la fin d'une analyse, cette cellule meure.

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