Gene reporter assay, kit and cells with improved sensitivity...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2667972

The present invention provides a reporter gene containing cell line with increased specificity and/or sensitivity for a particular extracellular signal of interest so that it can be used in a gene-reporter assay to accurately determine the presence and/or level of the extracellular signal of interest in the presence of other extracellular signals that are capable of activating the same signal transduction pathway as the extracellular signal of interest or that are capable of activating another signal transduction pathway capable of modulating the transcription of the reporter gene.

Cette invention concerne un gène rapporteur renfermant une lignée cellulaire à spécificité et/ou sensibilité accrues pour un signal extracellulaire d'intérêt de sorte qu'il peut s'utiliser dans une analyse de gène rapporteur pour déterminer avec précision la présence et/ou le niveau du signal extracellulaire d'intérêt en présence d'autres signaux extracellulaires, lesquels sont capables d'activer la même voie de transduction de signal que le signal extracellulaire d'intérêt ou bien une autre voie de transduction de signal capable de moduler la transcription du gène rapporteur.

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