C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2293734
The present invention provides a gene search method comprising P-element insertion and being capable of efficiently identifying a novel gene regulating various biological functions of Drosophila and specifying a function corresponding to the novel gene. An unknown gene of Drosophila can be searched by the method comprising a step of inserting in the genome of Drosophila a gene search vector carrying two sets of an expression regulatory sequence comprising UAS sequence for GAL4 transcription factor GAL4 and a promoter sequence, as integrated in the P-element sequence in such a manner that their downstreams are in opposite directions, mating the vector- inserted Drosophila with a Drosophila expressing the GAL4 to create progeny individuals, and identifying a vector-inserted line with a phenotype different from those of wild-type Drosophila, and determining the nucleotide sequence of the gene for the mutant phenotype.
Japan Science And Technology Corporation
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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