Gene signature

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C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C07H 21/04 (2006.01) C07K 14/47 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2153480

A 3'-directed cDNA library which accurately reflects the abundance ratio of mRNA in a cell has been prepared from various human tissues, and sequencing of the cDNAs contained in the library has been conducted to examine the incidence of each cDNA in each tissue. As each cDNA has expression information with each tissue corresponding to the mRNA concentration, these cDNAs are usable as a probe or primer for detecting cell anomaly or discriminating cells. The cloned gene can produce proteins utilizable as a medicine or the like.

On a préparé une banque d'ADNc à spécificité 3' reflétant précisément le rapport d'abondance de l'ARNn dans une cellule à partir de divers tissus humains, et on a procédé au séquençage des ADNc contenus dans la banque de manière à étudier l'incidence de chaque ADNc dans chaque tissu. Chaque ADNc comprenant des informations d'expression correspondant à la concentration d'ARNm, ces ADNc peuvent être utilisés en tant que sonde ou amorce pour détecter des anomalies cellulaires ou isoler des cellules. Le gène cloné peut produire des protéines utilisables sous forme de médicament ou analogue.

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