Gene silencing materials and methods

Details Gene silencing materials and methods Gene silencing materials and methods

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 3/00 (2006.01)

Patent

CA 2301894

Disclosed are methods for silencing a target nucleotide sequence (preferably representing one or more endogenous genes, preferably in a systemic fashion) which is present in a first part of the plant, which method comprises transiently introducing into the cytoplasm of a cell in a second part of the plant, which cell comprises a nucleic acid encoding the target sequence and which is remote from said first part of the plant, a nucleic acid construct.

On décrit des procédés destinés à rendre silencieuse une séquence nucléotidique cible (représentant, de préférence de manière systémique, un ou plusieurs gènes endogènes) présente dans la première partie d'une plante. Le procédé consiste à introduire, de manière transitoire, une construction d'acide nucléique dans le cytoplasme d'une cellule située dans une deuxième partie de la plante, ladite cellule comprenant un acide nucléique codant la séquence nucléotidique et ladite cellule étant éloignée de la première partie de la plante.

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