C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2335651
The present invention relates inter alia, to a method of initiating transcription of a target gene in a eukaryotic cell comprising: (a) providing a eukaryotic cell which is capable of producing a response protein; and (b) inserting into the genome of said cell a polynucleotide defining an inducible promoter sequence operably linked to and capable when induced of initiating transcription of said target gene; and (c) applying to said cell a chemical inducer capable of binding to said response protein whereby said chemical inducer binds to said response protein to form an inducing complex which binds to and induces said inducible promoter thereby initiating transcription of said target gene.
L'invention a trait, entre autres, à un procédé d'initiation de transcription d'un gène cible dans une cellule eucaryote, qui comprend les étapes consistant à: (a) prévoir une cellule eucaryote capable de produire une protéine de réaction; (b) insérer dans le génome de ladite cellule un polynucléotide définissant une séquence de promoteur inductible liée de façon exploitable audit gène cible, et capable après induction d'initier la transcription de celui-ci; et (c) appliquer à ladite cellule un inducteur chimique capable de se lier à ladite protéine de réaction, ce qui entraîne la liaison dudit inducteur chimique à ladite protéine de réaction pour former un complexe inducteur qui se lie audit promoteur inductible, et déclenche ainsi l'initiation de transcription dudit gène cible.
Fray Rupert George
Jepson Ian
Martinez Alberto
Na
Syngenta Limited
Zeneca Limited
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