Gene targeting methods and vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01) A01K 67/00 (2006.01) A01K 67/033 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2463233

Methods and vectors are provided for the specific alteration of particular genetic loci in eukaryotic cells. One method includes the utilization of positive-positive selection (PPS) DNA vectors for the purpose of creating and identifying cells which have vector sequences integrated into the host cell genome via site-specific homologous recombination. The procedure also comprises the utilization of sequences encoding in vivo detectable markers for the identification of cells which have exogenous vector sequences integrated into the genome of the host cell, either via site-specific homologous recombination or nonhomologous recombination or insertion. The invention also includes vectors for creating modifications in eukaryotic cells.

L'invention concerne des procédés et des vecteurs dont des sites génétiques particuliers sont spécifiquement altérés dans des cellules eucaryotes. Un procédé consiste à utiliser des vecteurs d'ADN à sélection positive-positive (PPS) en vue de créer et d'identifier des cellules qui présentent des séquences de vecteur intégrées dans le génome cellulaire hôte via la recombinaison homologue site-spécifique. Le procédé consiste en outre à utiliser des séquences codant des marqueurs détectables in vivo en vue de l'identification de cellules présentant des séquences de vecteurs hexogènes intégrées dans le génome de la cellule hôte, soit via la recombinaison homologue site-spécifique, soit via la recombinaison non homologue, soit encore via l'insertion. L'invention concerne également des vecteurs permettant de créer des modifications au sein des cellules eucaryotes.

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