Gene therapy of tumors using non-viral delivery system

A - Human Necessities – 61 – K

Patent

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Details

A61K 48/00 (2006.01) A61K 9/127 (2006.01) A61K 38/17 (2006.01) C12N 5/00 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2321972

The present invention provides a pharmaceutical composition comprising: (a) cationic lipids, wherein said lipids are a liposomal mixture of a diacyl-ethyl- phosphocholine and 1,2-diacyl-sn-glycero-3-phosphoethanolamine; and (b) a plasmid cDNA sequence encoding a protein having tumor suppressor or pro- apoptotic activity. This composition has a high gene transfection efficiency at non-toxic doses and is designed to transfect human bronchial premalignant lesions and early endobronchial malignancies. Also provided is a method of treating a cancerous or pre-cancerous condition of the respiratory tract in an individual in need of such treatment, comprising the step of administering to said individual a pharmacologically effective dose of a pharmaceutical composition comprising: (a) cationic lipids, wherein said lipids are a liposomal mixture of a diacyl-ethyl-phosphocholine and 1,2-diacyl-sn-glycero-3- phosphoethanolamine; and (b) a plasmid cDNA sequence encoding a protein having tumor suppressor or pro-apoptotic activity.

L'invention concerne une composition pharmaceutique comprenant: (a) des lipides cationiques, ces lipides étant formés d'un mélange liposomique d'une diacyl-éthyl-phosphocholine et d'une 1,2-diacyl-sn-glycéro-3-phosphoéthanolamine; et (b) une séquence d'ADN complémentaire de plasmide codant une protéine possédant une activité de suppresseur de tumeur ou pro-apoptotique. Cette composition présente une grande efficacité de transfection génique à doses non toxiques et est conçue pour transfecter des lésions précancéreuses bronchiques humaines et des affections malignes endobronchiques précoces. L'invention concerne également une méthode de traitement d'une affection cancéreuse ou précancéreuse des voies respiratoires chez un individu nécessitant un tel traitement, la méthode consistant à administrer à cet individu une dose efficace sur le plan pharmacologique d'une composition pharmaceutique comprenant: (a) des lipides cationiques, ces lipides étant formés d'un mélange liposomique d'une diacyl-éthyl-phosphocholine et d'une 1,2-diacyl-sn-glycér-3-phosphoéthanolamine; et (b) une séquence d'ADN complémentaire de plasmide codant une protéine possédant une activité de suppresseur de tumeur ou pro-apoptotique.

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