Gene-trapping construct for the identification and isolation...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/65 (2006.01) C12N 5/16 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2274092

The invention relates to a gene trap construct containing a first reporter gene, which, after activation, can activate a second reporter gene, and the use of this gene trap construct for identification and/or isolation of genes, in particular transiently expressed genes. The invention relates further to a cell, preferably a mammal cell, containing the above-mentioned gene trap construct. The invention relates furthermore to the use of this mammal cell for identification and/or isolation of genes, in particular transient genes. In addition, the invention relates to a vector containing the above- mentioned gene trap construction, as well as a kit for identifying and/or isolating genes, in particular transient genes, which contains at least the above-mentioned gene trap construct or the above-mentioned vector. Finally, the invention relates to a method for the identification and/or isolation of genes, in particular transient genes.

La présente invention concerne un piège à gènes obtenu par recombinaison, contenant un premier gène marqueur qui, après activation, peut activer un deuxième gène marqueur. L'invention concerne également l'utilisation de ce piège à gènes pour identifier et/ou isoler des gènes, notamment des gènes exprimés de façon transitoire. La présente invention concerne en outre une cellule, de préférence une cellule de mammifère, contenant ledit piège à gènes. Elle concerne également l'utilisation de cette cellule pour identifier et/ou isoler des gènes, notamment des gènes transitoires. Elle concerne par ailleurs un vecteur contenant ledit piège à gènes, ainsi qu'un kit pour identifier et/ou isoler des gènes, notamment des gènes transitoires, ledit kit contenant ledit piège à gènes et ledit vecteur. L'invention concerne enfin un procédé pour identifier et/ou isoler des gènes, notamment des gènes transitoires.

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