General buffer for restriction endonucleases

Details General buffer for restriction endonucleases General buffer for restriction endonucleases

C - Chemistry, Metallurgy

12

N

C12N 9/16 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2089118

ABSTRACT Restriction endonuclease buffer compositions which are suitable for use with essentially all restriction endonucleases. The compositions comprise Tris-HCl, MgCl2, and at least one of KCl, beta-mercaptoethanol and deoxyribonucleoside triphosphate. The buffer compositions provide efficient restriction of DNA using a wide variety of restriction endonucleases, regardless of the specific buffer requirements of the restriction endonuclease when using conventional restriction buffers.

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