Generation and expansion of lak cells

Details Generation and expansion of lak cells Generation and expansion of lak cells

C - Chemistry, Metallurgy

12

N

195/46

C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2015294

17 Abstract of the Disclosure Process for generating and expanding cells having LAK cell activity comprises (a) treating human PBMC to deplete monocytes, (b) culturing the resulting peripheral blood lymphocytes in medium containing IL-2 and human plasma or serum to generate LAK activity and (c) expanding the cells by culturing at low cell density in medium containing IL-2 and human plasma or serum in a culture bag made from an organic polymeric, oxygen permeable film. Monocytes are depleted by treating the PBMC with an ester of an L-amino acid such as phenylalanine methyl ester.

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