Generation and selection of novel dna-binding proteins and...

Details Generation and selection of novel dna-binding proteins and... Generation and selection of novel dna-binding proteins and...

C - Chemistry, Metallurgy

12

N

C12N 15/69 (2006.01) C07K 14/00 (2006.01) C07K 14/005 (2006.01) C07K 14/435 (2006.01) C07K 14/81 (2006.01) C12N 1/21 (2006.01) C12N 15/10 (2006.01) C12N 15/65 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12N 15/73 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2045516

2045516 9007862 PCTABS00001 Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding proteins and selection for proteins binding the desired target DNA sequence. A novel selection vector is used to reduce artifacts. Heterooligomeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.

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