Generation of dendritic cells from cd34+ precursors

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2496502

The present invention relates to a method for inducing development of dendritic cells from CD34+ precursor cells. More particularly, the present invention relates to a method of differentiating CD34+ precursor cells into myeloid- and or lymphoid-dendritic cells. The present invention provides a protocol for the development of dendritic cells from a biological sampleinter alia cord blood, bone marrow or peripheral blood CD34+ stem cells. The dendritic cells of the present invention are useful as therapeutic cellular agents such as in the development of vaccines and in modulating immunological responsiveness. More particularly, the present invention provides compositions which can be used for inducing a protective immune response in a subject against inter alia pathogenic infections, autoimmune diseases and cancer.

La présente invention concerne un procédé permettant d'induire le développement de cellules dendritiques à partir de cellules précurseurs CD34?+¿. L'invention concerne plus particulièrement un procédé permettant de différentier les cellules précurseurs CD34?+¿ en cellules dendritiques myéloïdes et/ou lymphoïdes. L'invention propose un protocole pour le développement de cellules dendritiques à partir d'un échantillon biologique prélevé notamment dans le sang ombilical, dans la moelle osseuse, ou dans les cellules souches CD34?+¿ du sang périphérique. Les cellules dendritiques de la présente invention conviennent comme agents cellulaires thérapeutiques, notamment pour la mise au point de vaccins et la modulation de la réponse immunologique. L'invention concerne plus particulièrement des compositions qu'il est possible d'utiliser pour induire une réponse immunitaire protectrice chez un sujet contre notamment des infections pathogènes, des affections auto-immunes et le cancer.

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