Generation of diversity in combinatorial libraries

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2279341

The invention concerns gene banks and combinatorial derivatives thereof, prepared using phagemid- or phage-display in combination with type IIS restriction enzymes and cosmid packaging; their use for the isolation of ligands, including enzyme inhibitors, agonists and antagonists for receptors, competitive binding peptides to a defined target, diagnostic ligands for diseases and autoimmune syndromes, including surveillance tools for immune status, post-translationally modified peptides, and such ligands generated by this technology.

L'invention concerne des banques de gènes et des dérivés combinatoires de ces dernières, préparés à l'aide d'affichage phagémides ou phages en combinaison avec des enzymes de restriction de type IIS et des encapsidations de cosmides. L'invention concerne aussi l'utilisation de ces produits pour isoler des ligands, y compris des inhibiteurs d'enzymes, des agonistes et des antagonistes pour des récepteurs, des peptides de liaison par compétition vers une cible définie, des ligands de diagnostique pour des maladies et des syndromes autoimmunitaires, y compris des outils de surveillance pour des états immunitaires, des peptides modifiés de manière post-translationnelle et des ligands de ce type générés par cette technologie.

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