Generation of multipotent central nervous system stem cells

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) C12N 5/0797 (2010.01) A61K 35/30 (2006.01) A61K 35/12 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2452160

Methods for generating various cellular phenotypes from central nervous system stem cells are disclosed. Cellular differentiation into phenotypes of organs and tissues within and outside of the central nervous system is induced by co- culture with target cell types or by soluble trophic factors and elements of the extracellular matrix. Established pluripotent CNS stem cell lines are also disclosed.

La présente invention concerne des procédés pour créer divers phénotypes cellulaires à partir de cellules souches du système nerveux central. Une différenciation cellulaire dans les phénotypes d'organes et de tissus au sein et à l'extérieur du système nerveux central est induite par une co-culture avec des types de cellules cibles ou au moyen de facteurs trophiques solubles et d'éléments de la matrice extracellulaire. La présente invention concerne également des lignées de cellules souches pluripotentes du système nerveux central.

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