Generation of neuronal cells from pluripotent stem cells

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0797 (2010.01) C12N 5/079 (2010.01) A61K 35/30 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2696801

The invention relates to in vitro methods for differentiating mammalian pluripotent stem cells into cells displaying a neuronal phenotype, more particularly into cortical-type neurons including inter alia pyramidal neurons and cortical inhibitory interneurons. The invention further encompasses so-obtained neuronal cells and cell population, compositions comprising such, and further uses of said neuronal cells and cell population.

L'invention porte sur des procédés in vitro pour différencier des cellules souches pluripotentes de mammifère en cellules affichant un phénotype neuronal, plus particulièrement en neurones de type corticaux comprenant entre autres des neurones pyramidaux et des interneurons inhibiteurs corticaux. L'invention porte en outre sur des cellules neuronales ainsi obtenues et sur une population de cellules, sur des compositions les comprenant, et en outre sur des utilisations desdites cellules neuronales et de ladite population de cellules.

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