Generation of recombinant genes in bacteriophages

Details Generation of recombinant genes in bacteriophages Generation of recombinant genes in bacteriophages

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/73 (2006.01)

Patent

CA 2573023

The present invention relates to in vivo methods for generating and detecting recombinant DNA sequences in bacteriophages or plasmids containing bacteriophage sequences, methods for generating hybrid genes and hybrid proteins encoded by these hybrid genes by the use of bacteriophages and plasmids containing bacteriophagesequences, bacteriophages and plasmids that can be used in these methods, and kits comprising appropriate bacterial host cells and bacteriophages or plasmids. DNA sequences for which these methods are relevant include protein-encoding and non-coding sequences.

L'invention concerne des méthodes in vivo de génération et de détection de séquences d'ADN recombinées dans des bactériophages ou des plasmides contenant des séquences de bactériophages. L'invention porte également sur des méthodes de génération de gènes hybrides et de protéines hybrides codées par ces gènes hybrides au moyen de bactériophages et de plasmides contenant des séquences de bactériophages, des bactériophages et des plasmides pouvant être utilisés dans ces méthodes, et sur des kits comprenant des cellules hôtes bactériophages et des bactériophages ou des plasmides appropriés. Des séquences d'ADN auxquelles ces méthodes sont applicables comprennent des séquences codant ou ne codant pas les protéines.

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