Generation of recombinant genes in prokaryotic cells by...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12N 1/20 (2006.01) C12N 15/65 (2006.01)

Patent

CA 2557490

The present invention relates in general to methods for generating and detecting recombinant DNA sequences in prokaryotic cells, in particular bacteria, by using two different extrachromosomal elements and extrachromosomal elements, in particular plasmids that can be used for conducting the inventive methods. DNA sequences for which these methods are relevant include protein-encoding and non-coding sequences.

L'invention concerne en général des procédés de production et de détection de séquences d'ADN de recombinaison dans des cellules procaryotes, en particulier des bactéries, par le biais de deux éléments extrachromosomiques différents et d'éléments extrachromosomiques, en particulier des plasmides, pouvant être utilisés dans la mise en oeuvre des procédés de l'invention. Les séquences d'ADN pour lesquelles ces procédés se révèlent utiles sont des séquences codant les protéines et des séquence non codantes.

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