C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2360342
The present invention provides a method for the rapid simultaneous production of a plurality of single-stranded DNA circles having a predetermined size and nucleotide sequence using pre-designed hairpin oligonucleotides containing complementary sequences for directing ligation to form dumbbell-shaped monomers followed by heat denaturation to yield single-stranded DNA circles.
La présente invention concerne un procédé de production rapide et simultanée de plusieurs molécules circulaires d'ADN à un seul brin possédant une dimension prédéfinie et un séquence nucléotidique utilisant des oligonucléotides à structure en épingle à cheveux types contenant des séquences complémentaires permettant à la ligation de former des monomères en forme d'haltère, puis par dénaturation thermique à produire des molécules circulaires d'ADN à un seul brin.
Molecular Staging Inc.
Smart & Biggar
LandOfFree
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