C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/86 (2006.01) C07K 14/11 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) C12N 9/12 (2006.01) C12N 15/87 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01)
Patent
CA 2253595
This invention provides a method of producing viral transfectants (viruses containing a heterologous nucleic acid) that eliminates the need for purified RNP complexes or for purified viral RNA polymerases. The method generally involves: (i) combining a ribonucleic acid (nucleic acid transcript) with an isolated nucleoprotein (NP) of the virus to form a synthetic ribonucleoprotein complex (RNP); (ii) transfecting a host cell with the synthetic ribonucleoprotein complex; and (iii) holding (culturing) the host cell under conditions permitting replication of the virus.
Cette invention se rapporte à un procédé pour produire des transfectants viraux (virus contenant un acide nucléique hétérologue) qui permet d'éliminer la nécessité de recourir à des complexes de ribonucléoprotéines (RNP) purifiés ou à des ARN-polymérases virales purifiées. Ce procédé, d'une façon générale, consiste: (i) à combiner un acide ribonucléique (acide nucléique de transcription) avec une nucléoprotéine (NP) isolée du virus, afin de former un complexe de ribonucléoprotéines (RNA) synthétique; (ii) à effectuer la transfection d'une cellule hôte avec ce complexe de ribonuncléoprotéines synthétique; et (iii) à maintenir (cultiver) cette cellule hôte dans des conditions permettant la réplication dudit virus.
Hemphill Mark L.
Shaw Michael W.
Xu Xiyan
Department Of Health And Human Services The Government Of The United States Of America Represented By Th
Finlayson & Singlehurst
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