Genes encoding carotenoid compounds

Details Genes encoding carotenoid compounds Genes encoding carotenoid compounds

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 9/10 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01) C12N 15/61 (2006.01) C12P 5/02 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2527557

A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and b-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

Dans cette invention, une famille multigénique biosynthétique caroténogénique est isolée à partir de la souche DC413 de Panteoa stewartii, l'organisation génétique de cette famille multigénique correspondant à : crtE-idi-crtX-crtY-crtI-crtB-crtZ. Les gènes contenus dans la famille multigénique codent géranyl-géranyl pyrophosphate (GGPP) synthétase (CrtE), isopentényl pyrophosphate isomérase (Idi), zéaxanthine glucosyl transférase (CrtX), lycopène cyclase (CrtY), phytoène désaturase (CrtI), phytoène synthase (CrtB), et .beta.-carotène hydroxylase (CrtZ). La famille multigénique, les gènes et leurs produits sont utilisés dans la conversion de la farnésyl-pyrophosphate en caroténoïdes. Par ailleurs, l'invention décrit les vecteurs contenant ces segments d'ADN, les cellules hôtes contenant ces vecteurs ainsi que les procédés de production de ces enzymes par la technologie de recombinaison de l'ADN dans des organismes hôtes transformés.

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