Genes encoding carotenoid compounds

Details Genes encoding carotenoid compounds Genes encoding carotenoid compounds

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12N 15/31 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2523789

Genes have been isolated from strain DC260, a member of the Enterobacteriaceae family, encoding geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), phytoene synthase (CrtB), phytoene desaturase (Crtl), lycopene cyclase (CrtY), .beta.-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl tranferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl phyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes b recombinant DNA technology in transformed host organisms are disclosed.

L'invention concerne des gènes isolés à partir de la souche DC260, un membre de la famille Enterobacteriaceae, codant pour l'activité de la géranyle-géranyle pyrophosphate (GGPP) synthétase (CrtE), de la phytoène synthase (CrtB), de la phytoène désaturase (Crtl), de la lycopène cyclase (CrtY), de la .beta.-carotène hydroxylase (CrtZ) et de la zeaxanthine glucoxyle transférase (CrtX). Ces gènes et leurs produits sont utiles pour la conversion de la farnésyle pyrophosphate en caroténoïdes. L'invention concerne également des vecteur contenant ces segments d'ADN, des cellules hôtes contenant ces vecteurs et des procédés destinés à la production de ces enzymes par technique de recombinaison de l'ADN dans des organismes hôtes transformés.

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