Genes encoding carotenoid compounds

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C07H 21/04 (2006.01) C12N 1/21 (2006.01) C12N 5/14 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/63 (2006.01) C12P 5/00 (2006.01) C12P 5/02 (2006.01) C12P 7/00 (2006.01)

Patent

CA 2522867

Genes have been isolated from Pectobacterium cypripedii encoding geranylgeranyl pyrophosphate (GGPP) synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), .beta.-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

Cette invention se rapporte à des gènes qui ont été isolés du <i>Pectobacterium cypripedii</i> et qui codent la géranylgéranyl pyrophosphate (GGPP) synthase (CrtE), la phytoène synthase (CrtB), la phytoène désaturase (CrtI), la lycopène cyclase (CrtY), la .szlig.-carotène hydroxylase (CrtZ) et la zéaxanthin glucosyl transférase (CrtX). Ces gènes et leurs produits sont utiles pour convertir le farnésyl pyrophosphate en caroténoïdes. Cette invention concerne des vecteurs contenant ces segments d'ADN, des cellules hôtes contenant ces vecteurs et des procédés pour produire ces enzymes par la technique d'ADN de recombinaison dans des organismes hôtes transformés.

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