Genes encoding nematode toxins

C - Chemistry – Metallurgy – 12 – N

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C12N 9/02 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2746766

Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5, 4, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:3, 1, 2, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.

La présente invention concerne des compositions et des méthodes conférant une activité nématicide à des bactéries, des végétaux, des tissus, des graines et des cellules végétales. La présente invention concerne des compositions incluant une séquence codant des polypeptides nématicides. Les séquences codantes peuvent être employées dans des constructions d'ADN ou des cassettes d'expression pour la transformation et l'expression chez des végétaux et des bactéries. Les compositions incluent également des bactéries, végétaux, tissus, graines et cellules végétales transformés. En particulier, la présente invention concerne des molécules d'acide nucléique nématicides isolées. De plus, la présente invention englobe les séquences d'acides aminés correspondant aux polynucléotides. En particulier, la présente invention concerne des molécules d'acide nucléique isolées incluant des séquences nucléotidiques codant la séquence d'acides aminés présentées dans les SEQ ID NO:5, 4, 8, 9, 13, 14, 47, 48 ou 49, les séquences nucléotidiques présentées dans les SEQ ID NO:3, 1, 2, 6, 7, 10, 11, 12, 15, 45 ou 46, ainsi que leurs variantes et leurs fragments.

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