Genes encoding proteins with pesticidal activity

Details Genes encoding proteins with pesticidal activity Genes encoding proteins with pesticidal activity

C - Chemistry, Metallurgy

12

N

C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01N 33/00 (2006.01) A01N 63/00 (2006.01) C07H 21/00 (2006.01) C07K 14/325 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12Q 1/25 (2006.01) G01N 33/68 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2490548

The invention provides .delta.-endotoxins that have been modified to have at least one cathepsin-sensitive proteolytic site and thus have improved pesticidal activity, nucleic acids encoding them and plants transformed with the nucleic acids. A method of increasing the pesticidal activity of a pesticidal protein is taught. The modified endotoxins can be used to control pests, especially plant pests.

La pr~sente invention concerne des acides nucl~iques, et des variants et des fragments de ces derniers, obtenus ~ partir de souches de Bacillus thuringiensis codant des .delta.-endotoxines qui poss­dent une activit~ pesticide ~ l'encontre d'insectes nuisibles. L'invention se rapporte ~galement ~ des acides nucl~iques mutag~nis~s qui ont ~t~ modifi~s pour coder des polypeptides pesticides tels que des endotoxines poss~dant une activit~ pesticide am~lior~e et/ou une sp~cificit~ d'insecte modifi~e. Des modes de r~alisation particuliers de l'invention se rapportent ~ des acides nucl~iques isol~s codant des prot~ines pesticides pouvant Útre optimis~es, et ~ des compositions pesticides, ~ des cassettes d'expression, et ~ des micro-organismes et ~ des plantes transform~s renfermant un acide nucl~ique de l'invention. Les compositions de l'invention trouvent leur utilisation dans des proc~d~s de lutte contre les insectes nuisibles, en particulier les parasites des v~g~taux.

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