Genes encoding proteolytic enzymes from aspargilli

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/62 (2006.01) A23J 3/16 (2006.01) A23J 3/34 (2006.01) A23L 1/29 (2006.01) A23L 1/305 (2006.01) A23L 2/02 (2006.01) A23L 2/84 (2006.01) C07K 16/40 (2006.01) C12H 1/00 (2006.01) C12N 15/57 (2006.01) C12N 15/63 (2006.01) C12Q 1/37 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2438680

The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

L'invention concerne des séquences de gènes qui viennent d'être identifiés qui codent de nouvelles protéases pouvant être obtenues à partir d'Aspergillus niger. L'invention concerne la séquence de gènes de pleine longueur, leurs séquences d'ADNc ainsi que la protéine fonctionnelle pleine longueur et les fragments de cette dernière. L'invention traite aussi de procédés utilisant ces enzymes dans des procédés industriels et des procédés de diagnostic des infections fongiques. L'invention concerne aussi des cellules transformées avec l'ADN selon l'invention et des cellules dans lesquelles une protéase selon l'invention est génétiquement modifiée pour améliorer ou réduire son activité et/ou son niveau d'expression.

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