Genes for s-adenosyl l-methionine: jasmonic acid carboxyl...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/10 (2006.01) C12N 15/54 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2412902

The present invention relates to a novel gene for S-adenosyl-L-methionine: jasmonic acid carboxyl methyltransferase, a novel jasmonic acid carboxyl methyltransferase protein synthesized therefrom, and a novel transgenic plant transformed with an expression vector containing said gene. It has been known that said enzyme synthesizes jasmonic acid methyl ester using jasmonic acid and S-adenosyl methionine as the substrate and jasmonic acid methyl ester is a compound mediating the defensive reactions upon invasion of phytopathogenic organisms and harmful insects as well as a compound for regulating the plant growth. By introducing said novel enzyme which is specifically expressed in flowers into the plant body, a transgenic plant which exhibits a resistance against phytopathogens, harmful insects and stresses without causing any adverse effect on the plant growth can be obtained.

La présente invention se rapporte à un nouveau gène codant la <I>S</I>-adénosyl-L-méthionine: carboxyle méthyltransférase d'acide jasmonique, à une nouvelle protéine de carboxyle méthyltransférase d'acide jasmonique synthétisée à partir du dit gène, et à une nouvelle plante transgénique transformée par un vecteur d'expression contenant ledit gène. On sait que cette enzyme synthétise l'ester de méthyle d'acide jasmonique en utilisant l'acide jasmonique et la <I>S</I>-adénosyle méthionine en tant que substrat et que l'ester de méthyle d'acide jasmonique est un composé médiateur des réactions de défense lors de l'invasion d'organismes phytopathogènes et d'insectes nuisibles ainsi qu'un composé de régulation de la croissance végétale. En introduisant dans le corps d'une plante cette nouvelle enzyme qui est exprimée spécifiquement dans des fleurs, il est possible d'obtenir une plante transgénique qui présente une certaine résistance aux phytopathogènes, aux insectes nuisibles et au stress sans générer aucun effet indésirable sur la croissance de la plante.

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