Genes from actinobacillus succinogenes 13oz (atcc 55618) for...

Details Genes from actinobacillus succinogenes 13oz (atcc 55618) for... Genes from actinobacillus succinogenes 13oz (atcc 55618) for...

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) A61K 39/02 (2006.01)

Patent

CA 2618694

Actinobacillus succinogenes genes and methods of using the genes in genetically engineered A. succinogenes so as to improve production of chemicals such as succinate, fumarate, malate, 5-aminolevulinate, 2- oxoglutarate, glutamate, and aspartate. The genetically engineered A. succinogenes strains are capable of overexpressing C4 enzymes. The genetically engineered A. succinogenes can have one or more gene knockouts or modifications that inhibit C3 enzymes. The fluxes supplying substrate to the C4 pathway can also be improved in some of the genetically engineered A. succinogenes.

L'invention concerne des gènes d'Actinobacillus succinogenes et des méthodes d'utilisation de ces gènes dans des souches d'A. succinogenes génétiquement modifiées en vue d'une amélioration de la production d'agents chimiques tels que le succinate, le fumarate, le malate, le 5-aminolévulinate, le 2-oxoglutarate, le glutamate et l'aspartate. Les souches d'A. succinogenes génétiquement modifiées sont capables de surexprimer les enzymes C4. Ces souches d'A. succinogenes génétiquement modifiées peuvent présenter une ou plusieurs inactivations ou modifications de gènes inhibant les enzymes C3. Les flux fournissant un substrat à la voie C4 peuvent également être améliorés dans certaines des souches d'A. succinogenes génétiquement modifiées.

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