Genes from propionibacterium freudenreichii encoding enzymes...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01) C12P 7/22 (2006.01) C12P 9/00 (2006.01) C12P 13/00 (2006.01) C12P 19/30 (2006.01) C12P 19/38 (2006.01) C12P 19/42 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2492332

Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encoding the four enzymes can be placed in Propionibacteria shuttle vectors and used to transform Propionibacteria hosts, in order to improve the production of vitamin B12 or a precursor thereof on an industrial scale during fermentation.

L'invention concerne quatre nouveaux gènes, et les enzymes codées par ceux-ci, qui sont présents dans l'espèce Propionibacterium freudenreichii, et qui sont impliqués dans au moins cinq étapes de la voie biosyntéthique de la vitamine B¿12?. Ces quatre enzymes sont: A: une acide cobyrinique a, c-diamide synthase; B: une enzyme bifonctionnelle, qui est une cobinamide kinase et une cobinamide phosphate guanyl transférase; C: une cobalamin 5-phosphate synthase; et D: une adénosyl transférase. Les gènes codant ces quatre enzymes peuvent être placés dans des vecteurs navettes de l'espèce Propionibacteria et utilisés pour la transformation d'hôtes de cette espèce afin d'améliorer la production de vitamine B¿12? ou d'un précurseur de celle-ci à une échelle industrielle pendant la fermentation.

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