Genes involved in cyclododecanone degradation pathway

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 5/06 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 9/18 (2006.01) C12N 15/52 (2006.01) C12N 15/55 (2006.01) C12P 7/26 (2006.01) C12P 7/40 (2006.01) C12P 7/42 (2006.01) C12P 7/44 (2006.01) C12P 7/64 (2006.01) C12P 17/08 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2389789

A 10 kb gene cluster has been isolated from Rhodococcus ruber SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.

L'invention concerne un regroupement de gènes de 10 kb isolé de Rhodococcus ruber SC1 comprenant des gènes codant pour des enzymes utiles pour la synthèse de diacide dodécanoïque à partir de cyclododécanone et d'autres intermédiaires cycliques. On a identifié les six cadres de lecture ouverts spécifiques qui sont associés à la biosynthèse de diacide dodécanoïque. En sus des substrats attendus, les enzymes de l'invention présentent une spécificité modérée pour des composés en C11-C15.

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