Genetic assay for cytochrome p450 iid bufuralol-1'- hydroxylase

Details Genetic assay for cytochrome p450 iid bufuralol-1'- hydroxylase Genetic assay for cytochrome p450 iid bufuralol-1'- hydroxylase

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2071636

Polymorphisms at positions 100, 271, 281, 294, 408, 506 or 1432 of the cytochrome P450 enzyme bufuralol-1'-hydroxylase are indicative of the extensive metaboliser/poor metaboliser phenotypes and can be detected using known methods such as am- plification of the DNA with the polymerase chain reaction, followed by digestion with a suitable restriction enzyme and analysis by gel electrophoresis. The EM/PM phenotype is relevant to calculating safe or effective drug doses for individuals.

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