Genetic constructs and compositions comprising rre and cte...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A61K 31/7088 (2006.01) A61K 39/21 (2006.01) A61K 48/00 (2006.01) A61P 31/18 (2006.01) C07K 14/16 (2006.01) C07K 19/00 (2006.01) C12N 5/00 (2006.01) C12N 15/48 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2506695

Genetic constructs that comprise a coding sequence for HIV-1 Rev, and a coding sequence for a desired protein are disclosed. Compositions that comprise at least two nucleic acid molecules in which at least one nucleic acid molecule comprises a coding sequence for HIV-1 Rev, and at least one nucleic acid molecule comprises a coding sequence for a desired protein are disclosed. In such genetic constructs and compositions comprising nucleic acid molecules, the coding sequence for the desired protein comprises at least a portion of coding sequence for an HIV structural protein that includes an RRE and at least one CTE. Methods of inducing an immune response against an immunogen in an individual, methods of delivering proteins to an individual and methods of producing proteins are also disclosed.

L'invention concerne des gènes hybrides qui comprennent une séquence codante pour Rev du VIH 1, et une séquence codante pour une protéine voulue. L'invention concerne des compositions comprenant au moins deux molécules d'acide nucléique ; au moins une molécule d'acide nucléique comprend une séquence codante pour Rev du VIH 1, et au moins une molécule d'acide nucléique comprend une séquence codante pour une protéine voulue. Dans ces gènes hybrides et compositions comprenant des molécules d'acide nucléique, la séquence codante pour la protéine voulue comprend au moins une partie d'une séquence codante pour une protéine structurale du VIH incluant un RRE et au moins un CTE. L'invention concerne aussi des procédés visant à induire chez un sujet une réaction immunitaire contre un immunogène, des procédés d'administration de protéines à un sujet et des procédés de production de protéines.

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