Genetic engineering process for the preparation of hirudins,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C07H 21/00 (2006.01) C07K 14/435 (2006.01) C07K 14/815 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/72 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1341124

It is possible to obtain hirudin in a genetic engineering process using a specific DNA sequence. The gene is advantageously synthesized in the form of several fragments which are ligated enzymatically to give two larger part sequences which are incorporated in hybrid plasmids and amplified in host organisms. After re-isolation of the part sequences, they are combined to give the total gene which is incorporated into a hybrid plasmid, and expression of the latter is induced in a host organism.

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