C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/35, 530/17.0
C12N 15/09 (2006.01) C07H 21/00 (2006.01) C07K 14/435 (2006.01) C07K 14/815 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/72 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1341124
It is possible to obtain hirudin in a genetic engineering process using a specific DNA sequence. The gene is advantageously synthesized in the form of several fragments which are ligated enzymatically to give two larger part sequences which are incorporated in hybrid plasmids and amplified in host organisms. After re-isolation of the part sequences, they are combined to give the total gene which is incorporated into a hybrid plasmid, and expression of the latter is induced in a host organism.
488468
Brauer Dieter
Engels Joachim
Habermann Paul
Uhlmann Eugen
Wengenmayer Friedrich
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Brauer Dieter
Engels Joachim
Habermann Paul
Schering Aktiengesellschaft
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