Genetic engineering process for the production of...

Details Genetic engineering process for the production of... Genetic engineering process for the production of...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C07K 14/195 (2006.01) C12N 1/21 (2006.01) C12N 9/80 (2006.01) C12N 11/06 (2006.01) C12N 15/70 (2006.01) C12P 7/40 (2006.01) C12P 13/02 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2074681

A genetic engineering process is disclosed for the production of S-(+)-2,2-dimethylcyclopropanecarboxamide. For this purpose, a new DNA that codes for a stereospecific hydrolase is isolated from a microorganism. This DNA is then ligated in an expression vector, and a hybrid plasmid results, which is used to transform microorganisms. These microorganisms are then able to biotransform the R-(-) isomer in racemic R,S-(~)-2,2-dimethylcyclopropanecarboxamide into R-(-)-2,2-dimethylcyclopropanecarboxylic acid. Optically active S-(-H)-2,2-dimethylcyclopropanecarboxamide is thus obtained.

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