Genetic engineering salt tolerance in crop plants

Details Genetic engineering salt tolerance in crop plants Genetic engineering salt tolerance in crop plants

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2323756

The invention is isolated nucleic acid molecules encoding Na+/H* exchanger polypeptides for extrusion of monovalent cations (preferably lithium ions and potassium ions, most preferably sodium ions) from the cytosol of cells to provide the cell with increased salt tolerance. In a preferred embodiment, the nucleic acid is obtained from Arabidopsis thaliana. Crop species transformed with the nucleic acid molecule are capable of surviving in soil with high salt levels that would normally inhibit growth of the crop species.

Dans la présente invention, des molécules d'acides nucléiques isolées codent des polypeptides échangeurs de Na+/H+ afin d'extraire des cations monovalents (de préférence des ions lithium et potassium, encore plus préférablement des ions sodium) du cytosol de cellules afin de conférer à ces cellules une meilleure tolérance au sel. Dans une réalisation préférée, l'acide nucléique est obtenu à partir d'Arabidopsis thaliana. Les espèces cultivées modifiées à l'aide de la molécule d'acide nucléique peuvent survivre dans des sols à forte teneur en sels, qui inhiberaient normalement leur croissance.

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