Genetic immunization with nonstructural proteins of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/51 (2006.01) A61K 39/29 (2006.01) C07K 14/18 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2318744

Nucleic acid molecules that comprise a hepatitis C nonstructural protein including specifically disclosed DNA sequences are disclosed. Pharmaceutical compositions that contain nucleic acid molecules comprising a hepatitis C nonstructural protein including a nucleotide sequence encoding NS3, NS4, or NS5, or a combination thereof, operably linked to regulatory elements functional in human cells are disclosed. Methods of immunizing individuals susceptible to or infected by hepatitis C virus comprising administering such pharmaceutical compositions are disclosed.

L'invention concerne une molécule d'acide nucléique comprenant une protéine non structurale de l'hépatite C, y compris en particulier des séquences d'ADN. L'invention concerne également des compositions pharmaceutiques contenant des molécules d'acide nucléique comprenant une protéine non structurale de l'hépatite C y compris une séquence nucléotidique codant NS3, NS4 ou NS5 ou une combinaison de ces dernières, liée de manière fonctionnelle à des éléments régulateurs fonctionnels dans des cellules humaines. L'invention concerne en outre des méthodes d'immunisation d'individus infectés ou susceptibles d'être infectés par le virus de l'hépatite C, les méthodes consistant à administrer les compositions pharmaceutiques.

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