C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) C07H 21/04 (2006.01) C12N 15/31 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2246259
A method, diagnostic sequences and primers are provided that are useful in the identification of the Escherichia coli 0157:H7 serotype. The method first involves the identification of a RAPD-amplified DNA fragment common to 0157:H7 Escherichia coli, the identification of the most conserved regions of that fragment, and the preparation of specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all 0157:H7 Escherichia coli.
L'invention porte sur un procédé, des séquences de diagnostic et des amorces utiles dans l'identification du sérotype 0157:H7de l'eschérichia coli. Ladite méthode consiste à identifier tout d'abord un fragment d'ADN amplifié par RAPD et commun aux escherichia coli 0157:H7, à identifier les régions les mieux conservées dudit fragment, puis à préparer des amorces spécifiques permettant de détecter la présence d'un marqueur dans le fragment. Lesdites amorces permettent donc l'identification de tous les escherichia coli 0157:H7.
E.i. Du Pont de Nemours And Company
Torys Llp
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