Genetic recombinant ecarin and process for preparing the same

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 9/64 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2452767

A modified ecarin protein capable of specifically activating prothrombin; and a process for producing this protein with the use of a gene modification technique which comprises: 1) the step wherein a transformant constructed by transforming a microbial or animal cell with an expression vector carrying a gene encoding ecarin integrated into the downstream of a promoter is cultured and the ecarin thus produced and accumulated in the culture medium or the transformant is collected; and 2) the step wherein the ecarin-containing solution thus collected and recovered is purified to give purified ecarin. According to this process, the modified ecarin protein can be produced on an industrial scale in practice.

L'invention concerne une protéine écarine génétiquement modifiée, capable d'activation spécifique sur la prothrombine, et un procédé d'élaboration correspondant qui repose sur une technique de modification génique, selon les étapes suivantes: 1) culture de transformant, issu de la transformation d'une cellule microbienne ou animale par un vecteur d'expression porteur de gène codant l'écarine intégrée en aval d'un promoteur, et collecte de l'écarine résultante accumulée à l'intérieur du milieu de culture ou du transformant; et 2) purification de la solution d'écarine ainsi recueillie, donnant une écarine purifiée. Dans la pratique, on peut élaborer ainsi une protéine écarine modifiée, à l'échelle industrielle.

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