Genetic selection of small molecule modulators of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2581341

The present invention provides a method of production and screening of small molecule modulation of inter-macromolecule interaction. The method involves providing a living cell containing a gene that directs expression of a gene product to be assayed for the ability to modulate inter-macromolecule interactions and an inter-macromolecule interaction whose interaction can be monitored. The inter-macromolecule interaction is monitored in the living cell to determine if the inter-macromolecule interaction is modulated in the living cell relative to another, otherwise similar living cell that lacks said gene product.

La présente invention concerne un procédé de production et d'analyse de la modulation à petites cellules de l'interaction inter-macromolécule. Ce procédé concerne une cellule vivante contenant un gène qui conduit l'expression d'un produit génique pour tester l'aptitude à moduler des interactions inter-macromolécule et une interaction macromolécule dont l'interaction peut être surveillée. L'interaction inter-macromolécule est surveillée dans la cellule vivante pour déterminer si l'interaction inter-macromolécule est modulée dans la cellule vivante par rapport à une autre cellule vivante similaire manquant de ce produit génique.

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