Genetic sequences having methyltransferase activity and uses...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) C12N 15/113 (2010.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01) C12N 15/54 (2006.01) C12N 15/82 (2006.01) C12P 17/02 (2006.01)

Patent

CA 2472161

The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine: anthocyanin 3' -O- methyltransferase or S-adenosyl-L-methionine: anthocyanin 3', 5'-O- methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

L'invention porte d'une manière générale sur une séquence génétique codant pour un polypeptide présentant l'activité de la méthyltransférase et sur l'utilisation de ladite séquence et/ou du polypeptide pour modifier une ou plusieurs des caractéristiques phénotypiques d'une plante. Plus particulièrement ladite méthyltransférase agit sur les flavonoïdes, spécialement du type anthocyanine. Encore plus particulièrement l'invention porte sur un polypeptide présentant l'activité de la <i>S</i>-adénosyl-L-méthionine: de l'anthocyanine 3' -<i>O</i>-méthyltransférase ou <i>S</i>-adénosyl-L-méthionine: anthocyanine 3', 5'-<i>O</i>-méthyltransférase. Plus particulièrement encore, l'invention porte sur une séquence génétique codant pour un polypeptide présentant l'activité de la méthyltransférase et dérivant du <i>Pétunia</i>, du <i>Torénia</i>, du<i>Fuchsia</i>, du <i>Plumbago</i>, ou de variétés botaniques apparentées. L'invention porte en outre sur des molécules antisens et sens correspondant à tout ou partie des susdites séquences génétiques, ainsi que sur des plantes modifiées génétiquement, et sur des fleures coupées ou leurs parties, extraits et tissus reproducteurs.

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